EMMA SOPs - Superovulation protocol
Inject 20-25 days-old females with 2.5-10 IU of PMSG between 3.00-6.00 pm (hormone dose depends on the age of the animal and on the mouse strain). 46-48 hours after PMSG administration, inject 2.5-5.0 IU of hCG (hormone dose depends on the age of the animal and on the mouse strain). The females should then be mated to selected males and plug checked the following morning (20 -24 hours post-hCG injection i.e. 0.5 day post coitum, dpc). Collect embryos from superovulated females 1.5 to 3.5 dpc.