EMMA - Protocols

All EMMA partners use validated protocols for mouse embryo and spermatozoa freezing techniques, in vitro fertilisation procedures and embryo transfers. The protocols provided for download on this website are practiced in EMMA training courses at the MRC-MGU (Harwell) and the CNRS-CDTA (Orléans) and are routinely used at Harwell and Orléans. The EMMA short training courses are intended to give animal technologists practical 'hands on' experience of the murine embryo and spermatozoa freezing techniques, simple and robust in vitro fertilization procedures and frozen embryo resuscitations. The protocol on the production of germ-free mice is provided by the Gulbenkian Institute which is offering the EMMA axenic (germ-free) service.

In addition to the validated protocols listed here, EMMA distribution centres will provide to customers ordering frozen embryos the centre specific validated thawing protocols.